effect on tumor metastasis is because of disrupting an interaction between CD47 and integrins. Results, cD47 Is Expressed on Solid Tumor Cells. The B6H12.2 antibody may affect the ability of CD47 to interact with these integrins, thereby inhibiting their ability to adhere and migrate ( 44 ). Purified tumor cells and TAMs were mixed in the presence of control IgG1 or anti-hCD47 mAbs and phagocytosis was evaluated by flow cytometry detection of GFP TAMs. A similar lack of toxicity was observed 1 wk after administering anti-mCD47 antibodies via intraperitoneal injection into tumor free balb/c mice ( Table S4 ). All animal procedures were approved by the Administrative Panel on Laboratory Animal Care at Stanford University. A single, substantially smaller, secondary lymph node was observed in one anti-hCD47 mAb-treated mouse ( Fig. ( A ) Representative images of NSG bone marrow-derived macrophages (bmdm) phagocytosing human tumor cells following treatment with the indicated antibody. 4 A, see Fig.
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Harsh, Matt van de Rijn, Nelson. ( C ) The total number of mice exhibiting lung micrometastases in each cohort. Given that TAMs are present in large numbers within tumors ( 46 it's possible that anti-CD47 antibody therapy has the potential to restore TAM immunosurveillance and fundamentally alter the role of macrophages in tumor biology. The total number of lung micrometastases is indicated. For ex vivo assays, TAMs (F4/80; eBioscience) and human tumor cells (GFP) were isolated by facs from single-cell suspensions prepared from large ( 1 cm3) subcutaneous xenograft tumors established in NSG mice.
Free textbooks written by more than 100 leading designers, bestselling authors, and Ivy League professors. We have assembled our textbooks in a gigantic encyclopedia, whose 4,000 pages cover the design of interactive products and services such as websites, household objects, smartphones. Adam Perer is an Assistant Research Professor at Carnegie Mellon University, where he is a member of the.